D-galactose induced inflammation lipid peroxidation and platelet activation in rats

Cytokine. 2014 Sep;69(1):150-3. doi: 10.1016/j.cyto.2014.05.006. Epub 2014 Jun 6.


Background: To investigate events possibly related to the development of D-galactose induced senescence, we examined whether 8-iso PGF(2α) formation, a marker of in vivo lipid peroxidation is altered and whether its biosynthesis is associated with 11-dehydro-TXB(2) excretion rate, as a marker of in vivo platelet activation. In this setting, we also investigated the relationship between proinflammatory mediators (IL-6 and TNF-α from one, and lipid peroxidation and platelet activation, from another aspect.

Methods and results: Forty animals were divided, depending on treatment with d-galactose into: placebo and D-galactose treated rats. 8-iso-PGF(2α), IL-6 and TNF-α were measured in plasma, while 11-dehydro-TXB(2) was determined in the urine after a six week treatment with d-galactose. Compared to placebo, d-galactose treated animals showed significantly higher levels of all measured parameters.

Conclusions: D-galactose induced changes in the rate of F(2)-isoprostane formation are associated with the changes in the excretion rate of 11-dehydro-TXB(2).

Keywords: Inflammation; Lipid peroxidation; Platelet activation; Rats; d-galactose induced senescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachidonic Acid / metabolism
  • Cellular Senescence
  • Dinoprost / analogs & derivatives*
  • Dinoprost / biosynthesis
  • Dinoprost / blood
  • Galactose / pharmacology*
  • Inflammation / chemically induced
  • Interleukin-6 / blood
  • Lipid Peroxidation / drug effects*
  • Male
  • Platelet Activation / immunology*
  • Rats
  • Rats, Wistar
  • Thromboxane B2 / analogs & derivatives*
  • Thromboxane B2 / metabolism
  • Thromboxane B2 / urine
  • Tumor Necrosis Factor-alpha / blood


  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • 8-epi-prostaglandin F2alpha
  • Arachidonic Acid
  • Thromboxane B2
  • 11-dehydro-thromboxane B2
  • Dinoprost
  • Galactose