A novel papillomavirus isolated from a nasal neoplasia in an Italian free-ranging chamois (Rupicapra r. rupicapra)

Vet Microbiol. 2014 Aug 6;172(1-2):108-19. doi: 10.1016/j.vetmic.2014.05.006. Epub 2014 May 14.

Abstract

Most amniotes are the hosts of many, distantly related papillomaviruses (PVs). Infection by PVs can be asymptomatic, or lead instead to benign or malignant lesions. However, PVs infecting animals and associated with malignancies are still largely understudied. In the present study, we communicate the complete genome of a novel PV found in a nasal neoplasia of a free-ranging alpine chamois (Rupicapra r. rupicapra) in an Italian national park. Long-PCR and cloning approaches followed for Sanger sequencing were used to identify the first PV found in chamois. The genome of the novel virus - RrupPV1 - of 7256 bp in length, presents the classical PV structure, and lacks the interE2-L2 region that hosts the E5 gene in AlphaPVs and in DeltaPVs. The nucleotide identity percentage of the L1 ORF, places RrupPV1 together with OaPV3 in the same genus. The latter is a PV isolated from a squamous cell carcinoma in sheep in Sardinia. Full-genome phylogenetic reconstructions suggest that these two viruses are sister taxa, and that both of them are very distantly related to any other known PV. Many cetartiodactyl species are infected by non-monophyletic PVs. Our results exemplify further the multiple links between the infection by certain, distantly related PVs and the development of diverse cancers in animals and highlight the need of a systematic search of oncogenic and non-oncogenic animal PVs.

Keywords: Animal viruses; Cancer; Co-evolution; Diversity; Infection; Papillomaviruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Genome, Viral*
  • Goat Diseases / pathology
  • Goat Diseases / virology*
  • Goats
  • Italy
  • Nasal Cavity / pathology
  • Nasal Cavity / virology
  • Nose Neoplasms / etiology
  • Nose Neoplasms / pathology
  • Nose Neoplasms / veterinary*
  • Nose Neoplasms / virology
  • Papillomaviridae / classification
  • Papillomaviridae / genetics*
  • Papillomaviridae / isolation & purification
  • Papillomavirus Infections / complications
  • Papillomavirus Infections / pathology
  • Papillomavirus Infections / veterinary*
  • Papillomavirus Infections / virology
  • Phylogeny*
  • Polymerase Chain Reaction
  • Rupicapra / virology*
  • Sequence Analysis, DNA