Mixed T helper cell signatures in chronic rhinosinusitis with and without polyps

PLoS One. 2014 Jun 9;9(6):e97581. doi: 10.1371/journal.pone.0097581. eCollection 2014.

Abstract

In chronic rhinosinusitis (CRS) different phenotypes have been reported based on cytokine profile and inflammatory cell patterns. The aim of this study was to characterize the intracytoplasmatic cytokines of T cells infiltrating the inflamed sinonasal mucosa.

Methods: Infiltrated T cells and tissue homogenates from sinonasal mucosal samples of 7 healthy subjects, 9 patients with CRS without nasal polyp (CRSsNP), 15 with CRS with nasal polyps (CRSwNP) and 5 cystic fibrosis patients (CF-NP) were analyzed for cytokine expression using flow cytometry and multiplex analysis respectively. Intracytoplasmic cytokinesin T cells were analyzed after stimulation of nasal polyps with Staphylococcus aureus enterotoxin B for 24 hours.

Results: The number of T cells per total living cells was significantly higher in patients with CRSwNP vs. CRSsNP and controls. 85% of the CD4(+) T cells showed to be memory T cells. The effector T cells present in all tissues have a predominant Th1 phenotype. Only in CRSwNP, a significant fraction of T cells produced the Th2 cytokines IL-4 and IL-5, while nasal polyps from CF patients were characterized by a higher CD4/CD8 T cell ratio and an increased number of Th17 cells. 24 h stimulation with SEB resulted in a significant induction of CD4(+) T cells producing IL-10 (Tr1 cells).

Conclusion: T cell cytokine patterns in healthy and inflamed sinonasal mucosa revealed that Th2 cells (IL-4 and IL-5 producing cells) are significantly increased in CRSwNP mucosal inflammation. Exposure to SEB stimulates Tr1 cells that may contribute to the Th2 bias in CRSwNP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / drug effects
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / drug effects
  • Case-Control Studies
  • Chronic Disease
  • Cytokines / metabolism
  • Enterotoxins / toxicity
  • Female
  • Humans
  • Male
  • Middle Aged
  • Nasal Polyps / complications*
  • Sinusitis / complications*
  • Sinusitis / immunology*
  • T-Lymphocytes, Helper-Inducer / cytology*
  • T-Lymphocytes, Helper-Inducer / drug effects
  • Young Adult

Substances

  • Cytokines
  • Enterotoxins
  • enterotoxin B, staphylococcal

Grant support

This project was supported by the Fund for Scientific Research Flanders (FWO-Vlaanderen – Projects 3G.0489. 08 to C.B., G.0642.10N to C.B) and The Interuniversity Attraction Poles Program (IUAP) – Belgian state – Belgian Science Policy P6/35 and P7/30. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.