Multidisciplinary analysis of a nontoxigenic Clostridium difficile strain with stable resistance to metronidazole

Antimicrob Agents Chemother. 2014 Aug;58(8):4957-60. doi: 10.1128/AAC.02350-14. Epub 2014 Jun 9.

Abstract

Stable resistance to metronidazole in a nontoxigenic Clostridium difficile strain was investigated at both the genomic and proteomic levels. Alterations in the metabolic pathway involving the pyruvate-ferredoxin oxidoreductase were found, suggesting that reduction of metronidazole, required for its activity, may be less efficient in this strain. Proteomic studies also showed a cellular response to oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Infective Agents / metabolism
  • Anti-Infective Agents / pharmacology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Clostridioides difficile / classification
  • Clostridioides difficile / drug effects
  • Clostridioides difficile / enzymology*
  • Clostridioides difficile / genetics*
  • Drug Resistance, Bacterial / genetics*
  • Enterocolitis, Pseudomembranous / drug therapy
  • Enterocolitis, Pseudomembranous / microbiology
  • Gene Expression
  • Genome, Bacterial*
  • Humans
  • Metabolic Networks and Pathways / genetics
  • Metronidazole / metabolism
  • Metronidazole / pharmacology
  • Microbial Sensitivity Tests
  • Oxidative Stress
  • Phylogeny
  • Proteomics
  • Pyruvate Synthase / genetics
  • Pyruvate Synthase / metabolism*
  • Ribotyping

Substances

  • Anti-Infective Agents
  • Bacterial Proteins
  • Metronidazole
  • Pyruvate Synthase