A comparative study of the effect of probiotics on cariogenic biofilm model for preventing dental caries

Arch Microbiol. 2014 Aug;196(8):601-9. doi: 10.1007/s00203-014-0998-7. Epub 2014 Jun 12.

Abstract

Dental caries is induced by oral biofilm containing Streptococcus mutans. Probiotic bacteria were mainly studied for effect on the gastrointestinal tract and have been known to promote human health. However, the information of probiotics for oral health has been lack yet. In this study, we investigated influence of various probiotics on oral bacteria or cariogenic biofilm and evaluated candidate probiotics for dental caries among them. The antimicrobial activity of the spent culture medium of probiotics for oral streptococci was performed. Probiotics were added during the biofilm formation with salivary bacteria including S. mutans. The oral biofilms were stained with a fluorescent dye and observed using the confocal laser scanning microscope. To count bacteria in the biofilm, the bacteria were plated on MSB and BHI agar plates after disrupting the biofilm and cultivated. Glucosyltransferases (gtfs) expression of S. mutans and integration of lactobacilli into the biofilm were evaluated by real-time RT-PCR. Among probiotics, Lactobacillus species strongly inhibited growth of oral streptococci. Moreover, Lactobacillus species strongly inhibited formation of cariogenic biofilm model. The expression of gtfs was significantly reduced by Lactobacillus rhamnosus. The integration of L. rhamnosus into the biofilm model did not exhibit. However, L. acidophilus and L casei integrated into the biofilm model. These results suggest that L. rhamnosus may inhibit oral biofilm formation by decreasing glucan production of S. mutans and antibacterial activity and did not integrate into oral biofilm, which can be a candidate for caries prevention strategy.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiosis
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Biofilms*
  • Culture Media
  • Dental Caries / microbiology
  • Dental Caries / prevention & control*
  • Enzyme Repression
  • Gene Expression Regulation, Bacterial
  • Glucosyltransferases / genetics
  • Glucosyltransferases / metabolism
  • Humans
  • Lactobacillus acidophilus / physiology
  • Lactobacillus casei / physiology
  • Lactobacillus rhamnosus / physiology*
  • Probiotics / therapeutic use*
  • Streptococcus gordonii / physiology
  • Streptococcus mutans / physiology*
  • Streptococcus oralis / physiology

Substances

  • Bacterial Proteins
  • Culture Media
  • Glucosyltransferases