The classical lancefield antigen of group a Streptococcus is a virulence determinant with implications for vaccine design

Cell Host Microbe. 2014 Jun 11;15(6):729-740. doi: 10.1016/j.chom.2014.05.009.


Group A Streptococcus (GAS) is a leading cause of infection-related mortality in humans. All GAS serotypes express the Lancefield group A carbohydrate (GAC), comprising a polyrhamnose backbone with an immunodominant N-acetylglucosamine (GlcNAc) side chain, which is the basis of rapid diagnostic tests. No biological function has been attributed to this conserved antigen. Here we identify and characterize the GAC biosynthesis genes, gacA through gacL. An isogenic mutant of the glycosyltransferase gacI, which is defective for GlcNAc side-chain addition, is attenuated for virulence in two infection models, in association with increased sensitivity to neutrophil killing, platelet-derived antimicrobials in serum, and the cathelicidin antimicrobial peptide LL-37. Antibodies to GAC lacking the GlcNAc side chain and containing only polyrhamnose promoted opsonophagocytic killing of multiple GAS serotypes and protected against systemic GAS challenge after passive immunization. Thus, the Lancefield antigen plays a functional role in GAS pathogenesis, and a deeper understanding of this unique polysaccharide has implications for vaccine development.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosamine / immunology
  • Acetylglucosamine / metabolism
  • Animals
  • Antibodies, Bacterial / immunology
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / metabolism
  • Antimicrobial Cationic Peptides
  • Bacterial Proteins / genetics
  • Carbohydrates / immunology
  • Cathelicidins / pharmacology
  • Epitopes
  • Female
  • Glycosyltransferases / metabolism
  • Host-Pathogen Interactions
  • Humans
  • Immunity, Innate
  • Male
  • Mice, Inbred Strains
  • Mutagenesis
  • Neutrophils / microbiology
  • Rabbits
  • Streptococcal Infections / immunology
  • Streptococcal Infections / virology*
  • Streptococcal Vaccines / genetics
  • Streptococcal Vaccines / immunology*
  • Streptococcus pyogenes / drug effects
  • Streptococcus pyogenes / immunology*
  • Streptococcus pyogenes / pathogenicity*
  • Virulence Factors / genetics


  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Antimicrobial Cationic Peptides
  • Bacterial Proteins
  • Carbohydrates
  • Cathelicidins
  • Epitopes
  • GacA protein, Bacteria
  • Streptococcal Vaccines
  • Virulence Factors
  • ropocamptide
  • Glycosyltransferases
  • Acetylglucosamine