CD27(+)CD56Bright natural killer cells may be involved in spontaneous clearance of acute hepatitis C in HIV-positive patients

AIDS. 2014 Aug 24;28(13):1879-84. doi: 10.1097/QAD.0000000000000355.

Abstract

Objective: The objective of this study was to analyse the potential role of CD27 in natural killer (NK) cell-mediated control of hepatitis C virus (HCV) infection in HIV-positive patients.

Design: Frequency of CD27-expressing CD56 NK cells was analysed in HIV mono-infected individuals and HIV-positive patients with acute or chronic hepatitis C. Anti-HCV activity of CD27(+) and CD27(-) NK cells was compared.

Methods: NK cell mediated inhibition of HCV replication was analysed using the HUH7 HCV Replicon model. NK cell phenotype and interferon (IFN) secretion was studied by flowcytometry.

Results: High frequency of CD27(+)CD56 NK cells is associated with spontaneous clearance of acute hepatitis C in HIV-positive patients. Accordingly, we found CD27(+)CD56 NK cells to display strong anti-HCV activity.

Conclusion: Our results underline the important role of NK cells in modulating outcome of HCV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • CD56 Antigen / analysis*
  • Female
  • Flow Cytometry
  • HIV Infections / complications*
  • Hepacivirus / immunology*
  • Hepatitis C / immunology*
  • Humans
  • Interferons / metabolism
  • Killer Cells, Natural / chemistry
  • Killer Cells, Natural / immunology*
  • Lymphocyte Subsets / chemistry
  • Lymphocyte Subsets / immunology*
  • Male
  • Middle Aged
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / analysis*

Substances

  • CD56 Antigen
  • NCAM1 protein, human
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Interferons