Abstract
A 30-min fluorogenic test was developed for differentiation of members of the Candida parapsilosis group from other Candida species commonly encountered in clinical material. The fluorogenic substrate, 4-methylumbelliferyl-beta-D-glucoside, was utilized to assay beta-glucosidase activity. A total of 50 C. parapsilosis isolates and 135 isolates of four other Candida species were tested. Assay sensitivity and specificity were 100 and 99.3%, respectively. The procedure was adapted for use with a spectrofluorometer.
MeSH terms
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Candida / classification*
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Candida / enzymology
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Candida / isolation & purification
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Glucosidases / analysis*
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Glucosides* / genetics
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Glucosides* / immunology*
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Glycosides* / genetics
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Glycosides* / immunology
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Hymecromone* / analogs & derivatives
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Hymecromone* / immunology*
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Predictive Value of Tests
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Spectrometry, Fluorescence
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Umbelliferones* / analogs & derivatives
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Umbelliferones* / immunology
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beta-Glucosidase / analysis*
Substances
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Glucosides
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Glycosides
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Umbelliferones
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4-methylumbelliferyl glucoside
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Hymecromone
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Glucosidases
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beta-Glucosidase