Using small angle solution scattering data in Xplor-NIH structure calculations

Prog Nucl Magn Reson Spectrosc. 2014 Jul;80:1-11. doi: 10.1016/j.pnmrs.2014.03.001. Epub 2014 Apr 3.


This contribution describes the use of small and wide angle X-ray and small angle neutron scattering for biomolecular structure calculation using the program Xplor-NIH, both with and without NMR data. The current algorithms used for calculating scattering curves are described, and the use of scattering data as a structural restraint is given concrete form as a fragment of an Xplor-NIH structure calculation script. We review five examples of the use of scattering data in structure calculation, including the treatment of single domain proteins, nucleic acids, structure determination of large proteins, and the use of ensemble representations to characterize small and large amplitude motions.

Keywords: NMR restraints; SANS; SAXS; Structure determination; WAXS.

Publication types

  • Research Support, N.I.H., Intramural
  • Review

MeSH terms

  • Animals
  • Humans
  • Macromolecular Substances / chemistry*
  • Models, Molecular
  • Molecular Structure
  • National Institutes of Health (U.S.)
  • Nuclear Magnetic Resonance, Biomolecular*
  • Scattering, Small Angle*
  • United States
  • X-Ray Diffraction / methods*


  • Macromolecular Substances