Staphylococcus Aureus Tetrapeptide With High Chemotactic Potency and Efficacy for Human Leukocytes

J Leukoc Biol. 1989 Feb;45(2):114-20. doi: 10.1002/jlb.45.2.114.

Abstract

A chemotactic tetrapeptide from culture fluids of Staphylococcus aureus was purified to homogeneity by reverse-phase high-pressure liquid chromatography. The peptide comprises equimolar methionine, leucine, isoleucine, and phenylalanine. It inhibited binding of fluoresceinated fMet-Leu-Phe-Lys to human monocytes, which showed that it interacted with the formyl-methionyl peptide receptor and suggested that it was a formyl-methionyl peptide. Based on a comparison of dose-response curves for inhibition of fluoresceinated fMet-Leu-Phe-Lys binding, the relative affinity of the peptide for the receptor was comparable to that of fMet-Leu-Phe-Lys. At optimal concentrations, chemotactic efficacy (percentage of monocytes migrating to the attractant) was 53 +/- 4%, in contrast to 36 +/- 3% for the reference attractant fMet-Leu-Phe. Since approximately 60% of human monocytes have formyl-peptide receptors, the bacterial peptide is capable of attracting all receptor-bearing monocytes.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / analysis
  • Binding, Competitive
  • Chemotactic Factors / isolation & purification
  • Chemotactic Factors / physiology*
  • Chemotaxis, Leukocyte / drug effects*
  • Fluorescein-5-isothiocyanate
  • Fluoresceins
  • Humans
  • Monocytes / metabolism
  • Monocytes / physiology
  • N-Formylmethionine Leucyl-Phenylalanine / analogs & derivatives
  • N-Formylmethionine Leucyl-Phenylalanine / metabolism
  • Oligopeptides / isolation & purification
  • Oligopeptides / physiology*
  • Receptors, Formyl Peptide
  • Receptors, Immunologic / drug effects
  • Staphylococcus aureus / analysis
  • Staphylococcus aureus / physiology*
  • Thiocyanates

Substances

  • Amino Acids
  • Chemotactic Factors
  • Fluoresceins
  • Oligopeptides
  • Receptors, Formyl Peptide
  • Receptors, Immunologic
  • Thiocyanates
  • chemotactic tetrapeptide
  • N-Formylmethionine Leucyl-Phenylalanine
  • N-formylmethionyl-leucyl-phenylalanyl-lysine
  • Fluorescein-5-isothiocyanate