Genome-wide profiling reveals a role for T-cell intracellular antigens TIA1 and TIAR in the control of translational specificity in HeLa cells

Isabel Carrascoso; Carmen Sánchez-Jiménez; José M Izquierdo

doi:10.1042/BJ20140227

Genome-wide profiling reveals a role for T-cell intracellular antigens TIA1 and TIAR in the control of translational specificity in HeLa cells

Biochem J. 2014 Jul 1;461(1):43-50. doi: 10.1042/BJ20140227.

Authors

Isabel Carrascoso¹, Carmen Sánchez-Jiménez¹, José M Izquierdo¹

Affiliation

¹ *Centro de Biología Molecular 'Severo Ochoa', Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid (CSIC/UAM), C/ Nicolás Cabrera 1, Cantoblanco, DP 28049 Madrid, Spain.

PMID: 24927121
DOI: 10.1042/BJ20140227

Abstract

TIA (T-cell intracellular antigens)-knockdown HeLa cells show an increase in ribosomes and translational machinery components. This increase correlates with specific changes in translationally up-regulated mRNAs involved in cell-cycle progression and DNA repair, as shown in polysomal profiling analysis. Our data support the hypothesis that a concerted activation of both global and selective translational rates leads to the transition to a more proliferative status in TIA-knockdown HeLa cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Profiling / methods*
Genome-Wide Association Study / methods*
HeLa Cells
Humans
Intracellular Fluid* / immunology
Poly(A)-Binding Proteins / genetics
Poly(A)-Binding Proteins / physiology*
RNA-Binding Proteins / genetics
RNA-Binding Proteins / physiology*
T-Cell Intracellular Antigen-1
T-Lymphocytes / chemistry
T-Lymphocytes / immunology*
Transcriptional Activation* / immunology

Substances

Poly(A)-Binding Proteins
RNA-Binding Proteins
T-Cell Intracellular Antigen-1
TIA1 protein, human
TIAL1 protein, human

Associated data

GEO/GSE54540