Sedimentation field flow fractionation monitoring of in vitro enrichment in cancer stem cells by specific serum-free culture medium

J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jul 15;963:40-6. doi: 10.1016/j.jchromb.2014.05.039. Epub 2014 May 27.

Abstract

The development of methods to enrich cell populations for cancer stem cells (CSC) is urgently needed to help understand tumor progression, therapeutic escape and to evaluate new drugs, in particular for colorectal cancer (CRC). In this work, we describe the in vitro use of OncoMiD for colon, a CRC-specific primary cell culture medium, to enrich CRC cell lines in CSC. Sedimentation field flow fractionation (SdFFF) was used to monitor the evolution of subpopulations composition. In these models, medium induced a loss of adherence properties associated with a balance between proliferation and apoptosis rates and, more important, an increased expression of relevant CSC markers, leading to specific SdFFF elution profile changes.

Keywords: Cancer stem cells; Cell enrichment; Colorectal cancer; Defined medium; Sedimentation field flow fractionation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Cell Adhesion
  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Separation / instrumentation*
  • Colorectal Neoplasms / metabolism
  • Colorectal Neoplasms / pathology*
  • Culture Media / metabolism*
  • Fractionation, Field Flow / instrumentation*
  • Humans
  • Neoplastic Stem Cells / cytology*
  • Neoplastic Stem Cells / metabolism
  • Neoplastic Stem Cells / pathology

Substances

  • Culture Media