Paenibacillus Lupini Sp. Nov., Isolated From Nodules of Lupinus Albus

Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3028-3033. doi: 10.1099/ijs.0.060830-0. Epub 2014 Jun 13.

Abstract

A bacterial strain designated RLAHU15(T) was isolated from root nodules of Lupinus albus in Spain. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate in the genus Paenibacillus, with its closest relatives being Paenibacillus catalpae D75(T), Paenibacillus glycanilyticus DS-1(T), Paenibacillus endophyticus PECAE04(T) and Paenibacillus xinjiangensis B538(T) with 98.8 %, 98.9 %, 97.4 % and 97.4 % similarity, respectively. DNA-DNA hybridization studies showed values lower than 45 % between the strain RLAHU15(T) and any of these species. The isolate was a Gram-stain positive, motile and sporulating rod. Catalase activity was weak and oxidase activity was positive. Casein and starch were hydrolysed but gelatin was not. Growth was supported by many carbohydrates and organic acids as carbon sources. MK-7 was the only menaquinone detected and anteiso-C15 : 0 and iso-C16 : 0 were the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids and an unidentified lipid. meso-Diaminopimelic acid was detected in the peptidoglycan. The DNA G+C content was 54.4 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain RLAHU15(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus lupini sp. nov. is proposed. The type strain is RLAHU15(T) ( = LMG 27296(T) = CECT 8235(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • DNA, Bacterial / genetics
  • Diaminopimelic Acid / chemistry
  • Fatty Acids / chemistry
  • Lupinus / microbiology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Paenibacillus / classification*
  • Paenibacillus / genetics
  • Paenibacillus / isolation & purification
  • Peptidoglycan / chemistry
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Root Nodules, Plant / microbiology*
  • Sequence Analysis, DNA
  • Spain
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Peptidoglycan
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • Diaminopimelic Acid
  • vitamin MK 7

Associated data

  • GENBANK/KF769449