A Nuclear Ubiquitin-Proteasome Pathway Targets the Inner Nuclear Membrane Protein Asi2 for Degradation

J Cell Sci. 2014 Aug 15;127(Pt 16):3603-13. doi: 10.1242/jcs.153163. Epub 2014 Jun 13.


The nuclear envelope consists of inner and outer nuclear membranes. Whereas the outer membrane is an extension of the endoplasmic reticulum, the inner nuclear membrane (INM) represents a unique membranous environment containing specific proteins. The mechanisms of integral INM protein degradation are unknown. Here, we investigated the turnover of Asi2, an integral INM protein in Saccharomyces cerevisiae. We report that Asi2 is degraded by the proteasome independently of the vacuole and that it exhibited a half-life of ∼45 min. Asi2 exhibits enhanced stability in mutants lacking the E2 ubiquitin conjugating enzymes Ubc6 or Ubc7, or the E3 ubiquitin ligase Doa10. Consistent with these data, Asi2 is post-translationally modified by poly-ubiquitylation in a Ubc7- and Doa10-dependent manner. Importantly Asi2 degradation is significantly reduced in a sts1-2 mutant that fails to accumulate proteasomes in the nucleus, indicating that Asi2 is degraded in the nucleus. Our results reveal a molecular pathway that affects the stability of integral proteins of the inner nuclear membrane and indicate that Asi2 is subject to protein quality control in the nucleus.

Keywords: ERAD; Nuclear membrane; Nuclear proteasome; Protein degradation; Ubiquitylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism
  • Cell Nucleus / enzymology*
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Nuclear Envelope / genetics
  • Nuclear Envelope / metabolism*
  • Proteasome Endopeptidase Complex / genetics
  • Proteasome Endopeptidase Complex / metabolism*
  • Proteolysis
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Ubiquitin-Conjugating Enzymes / genetics
  • Ubiquitin-Conjugating Enzymes / metabolism
  • Ubiquitins / metabolism*


  • Adaptor Proteins, Signal Transducing
  • Asi2 protein, S cerevisiae
  • DOA1 protein, S cerevisiae
  • Membrane Proteins
  • Saccharomyces cerevisiae Proteins
  • Ubiquitins
  • UBC6 protein, S cerevisiae
  • UBC7 protein, S cerevisiae
  • Ubiquitin-Conjugating Enzymes
  • Proteasome Endopeptidase Complex