The ability of a ternary complex to form over the serum response element correlates with serum inducibility of the human c-fos promoter

Cell. 1989 Feb 24;56(4):563-72. doi: 10.1016/0092-8674(89)90579-5.


Rapid induction of c-fos transcription by serum and phorbol esters requires the serum response element (SRE). The SRE contains a 20 bp element of interrupted dyad symmetry (DSE) that is bound by p67/SRF. We have identified a hitherto unrecognized protein with an apparent size of 62 kd. This novel component, p62, is shown to be an integral but physically separable part of a ternary complex formed with p67/SRF and the SRE. Alone, p62 fails to bind the SRE but requires DSE-bound p67/SRF and sequences both within and outside the DSE for its interaction with DNA. In vivo, the response of the c-fos promoter to serum is severely impaired by mutations that abolish ternary complex formation in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line
  • Culture Media
  • DNA-Binding Proteins / physiology*
  • Gene Expression Regulation*
  • Macromolecular Substances
  • Methylation
  • Molecular Weight
  • Promoter Regions, Genetic*
  • Protein Binding
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-fos
  • Regulatory Sequences, Nucleic Acid*
  • Structure-Activity Relationship
  • Transcription Factors / physiology*
  • Transcription, Genetic*


  • Culture Media
  • DNA-Binding Proteins
  • Macromolecular Substances
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-fos
  • Transcription Factors