Healing effect of sea buckthorn, olive oil, and their mixture on full-thickness burn wounds

Adv Skin Wound Care. 2014 Jul;27(7):317-23. doi: 10.1097/01.ASW.0000451061.85540.f9.


Objective: The purpose of this study is to evaluate the healing effect of silver sulfadiazine (SSD), sea buckthorn, olive oil, and 5% sea buckthorn and olive oil mixture on full-thickness burn wounds with respect to both gross and histopathologic features.

Methods: Full-thickness burns were induced on 60 rats; the rats were then were divided into 5 groups and treated with sea buckthorn, olive oil, a 5% sea buckthorn/olive oil mixture, SSD, and normal saline (control). They were observed for 28 days, and the wounds' healing process was evaluated.

Results: Wound contraction occurred faster in sea buckthorn, olive oil, and the sea buckthorn/olive oil mixture groups compared with the SSD and control groups. The volume of the exudates was controlled more effectively in wounds treated with the sea buckthorn/olive oil mixture. Purulent exudates were observed in the control group, but the others did not show infection. The group treated with sea buckthorn/olive oil mixture revealed more developed re-epithelialization with continuous basement membrane with a mature granulation tissue, whereas the SSD-treated group showed ulceration, necrosis, and immature granulation. The results show that sea buckthorn and olive oil individually are proper dressing for burn wounds and that they also show a synergetic effect when they are used together.

Conclusion: A sea buckthorn and olive oil mixture could be considered as an alternative dressing for full-thickness burns because of improved wound healing characteristics and antibacterial property.

Publication types

  • Comparative Study

MeSH terms

  • Administration, Cutaneous
  • Animals
  • Biopsy, Needle
  • Burns / drug therapy*
  • Burns / pathology
  • Disease Models, Animal
  • Drug Therapy, Combination
  • Hippophae*
  • Immunohistochemistry
  • Male
  • Olive Oil*
  • Phytochemicals / pharmacology
  • Phytotherapy / methods*
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Severity of Illness Index
  • Statistics, Nonparametric
  • Treatment Outcome
  • Wound Healing / drug effects*
  • Wound Healing / physiology


  • Olive Oil
  • Phytochemicals