A golden gate modular cloning toolbox for plants

ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. Epub 2014 Feb 20.

Abstract

Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.

Keywords: Agrobacterium tumefaciens; gene assembly; genetic engineering; molecular cloning; plant biotechnology; vector construction.

MeSH terms

  • Agrobacterium tumefaciens / genetics
  • Cloning, Molecular / methods*
  • Genetic Engineering / methods*
  • Genetic Vectors / genetics*
  • Models, Genetic
  • Synthetic Biology / methods*
  • Tobacco / genetics