Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 3 (11), 839-43

A Golden Gate Modular Cloning Toolbox for Plants


A Golden Gate Modular Cloning Toolbox for Plants

Carola Engler et al. ACS Synth Biol.


Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.

Keywords: Agrobacterium tumefaciens; gene assembly; genetic engineering; molecular cloning; plant biotechnology; vector construction.

Similar articles

See all similar articles

Cited by 99 articles

See all "Cited by" articles

MeSH terms

LinkOut - more resources