Covalent, sequence-specific attachment of long DNA molecules to a surface using DNA-templated click chemistry

Chem Commun (Camb). 2014 Aug 4;50(60):8131-3. doi: 10.1039/c4cc02900c.

Abstract

We present a novel method that covalently and sequence-specifically attaches long DNA molecules to a surface that is compatible with high-resolution atomic force microscopy (AFM) imaging. Surfaces prepared with this approach are ideally suited for performing biophysical experiments on single DNA molecules.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Pairing
  • Click Chemistry / methods*
  • Copper / chemistry*
  • DNA / chemistry*
  • Microscopy, Atomic Force
  • Surface Properties

Substances

  • Copper
  • DNA