Electrophysiological Studies of Voltage-Gated Sodium Channels Using QPatch HT, an Automated Patch-Clamp System

Curr Protoc Pharmacol. 2014 Jun 16;65:11.14.1-45. doi: 10.1002/0471141755.ph1114s65.

Abstract

Voltage-gated sodium (Na(v)) channels are highly sensitive to membrane potential and have fast gating kinetics. Patch clamp electrophysiology has long been the gold standard for studying these channels. Combining high throughput with high information content/accuracy, automated patch clamp technologies have emerged as critical tools in ion channel drug discovery. Described in this unit is the use of QPatch, one of the automated patch clamp systems, to study Na(v) channel function and pharmacology.

Keywords: HTS; Nav channel; Nav1.7; QPatch; patch clamp; voltage-gated sodium channel.

MeSH terms

  • Animals
  • Automation, Laboratory
  • Electrophysiological Phenomena
  • Humans
  • Patch-Clamp Techniques / methods*
  • Patch-Clamp Techniques / standards
  • Voltage-Gated Sodium Channels / drug effects
  • Voltage-Gated Sodium Channels / physiology*

Substances

  • Voltage-Gated Sodium Channels