Inhibitory activity of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (higher Basidiomycetes) on transformed cells by human papillomavirus

Int J Med Mushrooms. 2014;16(2):179-87. doi: 10.1615/intjmedmushr.v16.i2.80.


In this study, we investigated the effects of the aqueous extracts of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum, obtained from three localities (China; and Morelos and Michoacan, Mexico) on cervical cells transformed by human papillomavirus (HeLa and SiHa) and C-33A cancer cells. The cells were plated in DMEM medium supplemented, and were incubated in the presence of different concentrations of G. lucidum for 24 h. Cell proliferation was determined by MTT colorimetric assay and viability by trypan blue assay. Inhibitory dose was determined (IC50) of the three different extracts of G. lucidum in the culture cell lines mentioned above. The apoptosis process was confirmed by nuclear DNA fragmentation and the cell cycle was determined by flow cytometry. The results showed that aqueous extracts G. lucidum obtained from three localities produced inhibition in the proliferation of VPH transformed cells; they also induced apoptosis and cell cycle arrest in HeLa, SiHa, and C-33A cancer cells. Therefore, it was found that aqueous extracts G. lucidum obtained from three different locations produced inhibitory effect on cancer cells and may have a potential therapeutic use for the prevention and treatment of this disease.

MeSH terms

  • Antiviral Agents / pharmacology*
  • Apoptosis / drug effects
  • Cell Cycle / drug effects
  • Cell Line, Transformed
  • Cell Proliferation / drug effects
  • Cell Transformation, Viral / drug effects
  • Drugs, Chinese Herbal / pharmacology*
  • Human papillomavirus 16 / drug effects*
  • Human papillomavirus 16 / physiology
  • Human papillomavirus 18 / drug effects*
  • Human papillomavirus 18 / physiology
  • Humans
  • Papillomavirus Infections / drug therapy
  • Papillomavirus Infections / physiopathology
  • Papillomavirus Infections / virology*
  • Plant Extracts / pharmacology*
  • Reishi / chemistry*


  • Antiviral Agents
  • Drugs, Chinese Herbal
  • Plant Extracts