Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag

Methods Mol Biol. 2014;1177:35-45. doi: 10.1007/978-1-4939-1034-2_3.

Abstract

Easy-to-perform and low-cost protein purification methods are in high demand for the mass production of commonly used enzymes that play an important role in bioeconomy. A low-cost and rapid recombinant protein purification system was developed using CBM3 (family 3 cellulose-binding module) as affinity tag. This protocol describes the purification of CBM3-fusion protein and tag-free protein expressed in Pichia pastoris using CBM3 as an affinity tag.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cellulose / chemistry*
  • Chromatography, Affinity / methods*
  • Escherichia coli
  • Molecular Biology / methods*
  • Pichia / chemistry
  • Protein Binding
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / isolation & purification*

Substances

  • Recombinant Proteins
  • Cellulose
  • microcrystalline cellulose