CPF-associated phosphatase activity opposes condensin-mediated chromosome condensation

PLoS Genet. 2014 Jun 19;10(6):e1004415. doi: 10.1371/journal.pgen.1004415. eCollection 2014 Jun.


Functional links connecting gene transcription and condensin-mediated chromosome condensation have been established in species ranging from prokaryotes to vertebrates. However, the exact nature of these links remains misunderstood. Here we show in fission yeast that the 3' end RNA processing factor Swd2.2, a component of the Cleavage and Polyadenylation Factor (CPF), is a negative regulator of condensin-mediated chromosome condensation. Lack of Swd2.2 does not affect the assembly of the CPF but reduces its association with chromatin. This causes only limited, context-dependent effects on gene expression and transcription termination. However, CPF-associated Swd2.2 is required for the association of Protein Phosphatase 1 PP1(Dis2) with chromatin, through an interaction with Ppn1, a protein that we identify as the fission yeast homologue of vertebrate PNUTS. We demonstrate that Swd2.2, Ppn1 and PP1Dis2 form an independent module within the CPF, which provides an essential function in the absence of the CPF-associated Ssu72 phosphatase. We show that Ppn1 and Ssu72, like Swd2.2, are also negative regulators of condensin-mediated chromosome condensation. We conclude that Swd2.2 opposes condensin-mediated chromosome condensation by facilitating the function of the two CPF-associated phosphatases PP1 and Ssu72.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Acid Anhydride Hydrolases / genetics
  • Adenosine Triphosphatases / genetics
  • Cell Cycle Proteins / metabolism
  • Chromatin / metabolism
  • DNA Polymerase III / metabolism
  • DNA-Binding Proteins / genetics
  • Histone-Lysine N-Methyltransferase / genetics*
  • Histones / metabolism
  • Multiprotein Complexes / genetics
  • Phosphoprotein Phosphatases / metabolism*
  • Phosphorylation
  • Protein Phosphatase 1 / metabolism*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Transcription Termination, Genetic
  • mRNA Cleavage and Polyadenylation Factors / metabolism*


  • Cell Cycle Proteins
  • Chromatin
  • DNA-Binding Proteins
  • Histones
  • Multiprotein Complexes
  • SSU72 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • condensin complexes
  • mRNA Cleavage and Polyadenylation Factors
  • Histone-Lysine N-Methyltransferase
  • SWD2 protein, S cerevisiae
  • DNA Polymerase III
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 1
  • Acid Anhydride Hydrolases
  • Ppn1 protein, S cerevisiae
  • Adenosine Triphosphatases