K-252a specifically inhibits the survival and morphological differentiation of NGF-dependent neurons in primary cultures of human dorsal root ganglia

Neurosci Lett. 1989 Jan 2;96(1):1-6. doi: 10.1016/0304-3940(89)90233-4.


We have measured neurofilament protein expression in cultures of human dorsal root ganglia (DRG) grown in both the presence and absence of nerve growth factor (NGF). Cultures established from DRG of 8-13 weeks gestation contained populations of both NGF-dependent and -independent neurofilament-positive cells. A recently described kinase inhibitor named K-252a inhibited NGF-dependent increases in neurofilament protein expression with half-maximal and maximal inhibition apparent at concentrations of 6 and 25 nM. At up to 50 nM, K-252a had no effect on neurofilament protein expression from NGF-independent neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbazoles / pharmacology*
  • Cell Differentiation / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Ganglia, Spinal / cytology*
  • Ganglia, Spinal / drug effects
  • Ganglia, Spinal / metabolism
  • Humans
  • Immunohistochemistry
  • Indole Alkaloids
  • Intermediate Filament Proteins / metabolism
  • Nerve Growth Factors / pharmacology*
  • Neurofilament Proteins


  • Carbazoles
  • Indole Alkaloids
  • Intermediate Filament Proteins
  • Nerve Growth Factors
  • Neurofilament Proteins
  • staurosporine aglycone