An optimized DNA extraction protocol for benthic Didymosphenia geminata

Noelia Mariel Uyua; Julieta Marina Manrique; Leandro Roberto Jones

doi:10.1016/j.mimet.2014.06.007

An optimized DNA extraction protocol for benthic Didymosphenia geminata

J Microbiol Methods. 2014 Sep:104:12-8. doi: 10.1016/j.mimet.2014.06.007. Epub 2014 Jun 16.

Authors

Noelia Mariel Uyua¹, Julieta Marina Manrique¹, Leandro Roberto Jones²

Affiliations

¹ Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Av. 9 de Julio y Belgrano s/n, (9100) Trelew, Chubut, Argentina.
² Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, Av. 9 de Julio y Belgrano s/n, (9100) Trelew, Chubut, Argentina. Electronic address: ljones@conicet.gov.ar.

PMID: 24946185
DOI: 10.1016/j.mimet.2014.06.007

Abstract

Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously described DNA extraction techniques.

Keywords: Benthic; DNA extraction; Didymosphenia; Invasive; PCR; Stalk.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Analytic Sample Preparation Methods / methods*
DNA / genetics
DNA / isolation & purification*
Diatoms / genetics*

Substances

DNA