A Non-Digestible Fraction of the Common Bean (Phaseolus Vulgaris L.) Induces Cell Cycle Arrest and Apoptosis During Early Carcinogenesis

Plant Foods Hum Nutr. 2014 Sep;69(3):248-54. doi: 10.1007/s11130-014-0428-7.

Abstract

We have previously demonstrated that the non-digestible fraction (NDF) from common cooked beans (P. vulgaris L., cv Negro 8025) inhibits azoxymethane (AOM)-induced colon cancer and influences the expression of genes involved in the induction of apoptosis and cell cycle arrest through the action of butyrate. The objective of this study was to identify cell cycle alterations and morphological changes induced by treatment with AOM and to examine the formation of colonic aberrant crypt foci (ACF) in male Sprague Dawley rats fed with these beans. Rats were fed control diets upon arrival and were randomly placed into four groups after one week of acclimatization: control, NDF (intragastric administration), NDF + AOM and AOM. Rats treated with NDF + AOM exhibited a significantly lower number of total colonic ACF with a notable increase in the number of cells present in the G1 phase (83.14%); a decreased proliferation index was observed in the NDF + AOM group when compared to AOM group. NDF + AOM also displayed a higher number of apoptotic cells compared to AOM group. NDF of cooked common beans inhibited colon carcinogenesis at an early stage by inducing cell cycle arrest of colon cells and morphological changes linked to apoptosis, thus confirming previous results obtained with gene expression studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aberrant Crypt Foci / chemically induced
  • Aberrant Crypt Foci / drug therapy
  • Animals
  • Apoptosis / drug effects*
  • Azoxymethane / toxicity
  • Carcinogenesis / drug effects*
  • Cell Cycle Checkpoints / drug effects*
  • Cell Line, Tumor
  • Colon / cytology
  • Colon / drug effects
  • Colon / metabolism
  • Colonic Neoplasms / drug therapy
  • Cooking
  • Dietary Carbohydrates / analysis
  • Dietary Fiber / analysis
  • Dietary Proteins / analysis
  • Digestion
  • Flavonoids / analysis
  • Male
  • Phaseolus / chemistry*
  • Plant Extracts / pharmacology*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Dietary Carbohydrates
  • Dietary Fiber
  • Dietary Proteins
  • Flavonoids
  • Plant Extracts
  • Azoxymethane