Effects of UV radiation on hatching, lipid peroxidation, and fatty acid composition in the copepod Paracyclopina nana

Comp Biochem Physiol C Toxicol Pharmacol. 2014 Sep;165:60-6. doi: 10.1016/j.cbpc.2014.06.001. Epub 2014 Jun 18.

Abstract

To evaluate the effects of UV radiation on the reproductive physiology and macromolecules in marine zooplankton, several doses of UV radiation were used to treat the copepod Paracyclopina nana, and we analyzed in vivo endpoints of their life cycle such as mortality and reproductive parameters with in vitro biochemical biomarkers such as reactive oxygen species (ROS), the modulated enzyme activity of glutathione S-transferase (GST) and superoxide dismutase (SOD), and the production of a byproduct of peroxidation (e.g. malonedialdehyde, MDA). After UV radiation, the survival rate of P. nana was significantly reduced. Also, egg sac damage and a reduction in the hatching rate of offspring were observed in UV-irradiated ovigerous females. According to the assessed biochemical parameters, we found dose-dependent increases in ROS levels and high levels of the lipid peroxidation decomposition product by 2 kJ m(-2), implying that P. nana was under off-balanced status by oxidative stress-mediated cellular damage. Antioxidant enzyme activities of GST and SOD increased over different doses of UV radiation. To measure UV-induced lipid peroxidation, we found a slight reduction in the composition of essential fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). These findings indicate that UV radiation can induce oxidative stress-triggered lipid peroxidation with modulation of antioxidant enzyme activity, leading to a significant effect on mortality and reproductive physiology (e.g. fecundity). These results demonstrate the involvement of UV radiation on essential fatty acids and its susceptibility to UV radiation in the copepod P. nana compared to other species.

Keywords: Copepod; Fatty acid composition; Lipid peroxidation; Oxidative stress; Paracyclopina nana; UV-B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Biomarkers / metabolism
  • Copepoda / metabolism
  • Copepoda / radiation effects*
  • Docosahexaenoic Acids / metabolism
  • Eicosapentaenoic Acid / metabolism
  • Fatty Acids / metabolism*
  • Female
  • Glutathione Transferase / metabolism
  • Lipid Peroxidation / radiation effects*
  • Oxidative Stress / radiation effects
  • Reactive Oxygen Species / metabolism
  • Reproduction / radiation effects*
  • Superoxide Dismutase / metabolism
  • Survival Rate
  • Ultraviolet Rays / adverse effects*

Substances

  • Antioxidants
  • Biomarkers
  • Fatty Acids
  • Reactive Oxygen Species
  • Docosahexaenoic Acids
  • Eicosapentaenoic Acid
  • Superoxide Dismutase
  • Glutathione Transferase