A Novel Fluid Resuscitation Protocol: Provide More Protection on Acute Kidney Injury During Septic Shock in Rats

Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. eCollection 2014.

Abstract

Objective: To investigate the therapeutic effects of a novel fluid resuscitation protocol (early fluid resuscitation plus 2% hydrogen inhalation) on acute kidney injury during septic shock induced by lipopolysaccharide in rats.

Methods: Sixty male Wistar rats were randomly divided into four groups (n = 15 per group): control group (C), septic shock group (S), septic shock with early fluid resuscitation group (R), and septic shock with early fluid resuscitation plus 2% hydrogen inhalation group (R+R+H2). The rats were ventilated, and a 2% hydrogen mixture was used in Group R+H2. Lipopolysaccharide (10 mg/kg) was administered to establish the septic shock model in rats and fluid resuscitation was performed in Groups R and R+R+H2.

Results: Fluid resuscitation with 2% hydrogen inhalation decreased serum creatinine, blood urea nitrogen, and neutrophil gelatinase-associated lipocalin. It also reduced oxidative stress injury and decreased renal tumor necrosis factor-α and interleukin-6 levels compared with fluid resuscitation alone.

Conclusion: Early fluid resuscitation plus 2% hydrogen inhalation provided more protection against AKI during septic shock.

Keywords: Septic shock; acute kidney injury; fluid resuscitation; hydrogen inhalation; inflammation; oxidative stress.