Miniaturized bronchoscopy enables unilateral investigation, application, and sampling in mice

Am J Respir Cell Mol Biol. 2014 Dec;51(6):730-7. doi: 10.1165/rcmb.2014-0052MA.


Lung diseases, including pneumonia and asthma, are among the most prevalent human disorders, and murine models have been established to investigate their pathobiology and develop novel treatment approaches. Whereas bronchoscopy is valuable for diagnostic and therapeutic procedures in patients, no equivalent for small rodents has been established. Here, we introduce a miniaturized video-bronchoscopy system offering new opportunities in experimental lung research. With an outer diameter of 0.75 mm, it is possible to advance the optics into the main bronchi of mice. An irrigation channel allows bronchoalveolar lavage and unilateral application of substances to one lung. Even a unilateral infection is possible, enabling researchers to use the contralateral lung as internal control.

Keywords: bronchoscopy; mouse; unilateral application in lung.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bronchoscopes*
  • Bronchoscopy / methods
  • Disease Models, Animal
  • Humans
  • Influenza A Virus, H1N1 Subtype / immunology
  • Lung / immunology
  • Lung / microbiology
  • Lung / pathology*
  • Macrophage-Activating Factors / pharmacology
  • Mice, Inbred C57BL
  • Neutrophil Infiltration
  • Orthomyxoviridae Infections / diagnosis
  • Orthomyxoviridae Infections / immunology
  • Pneumonia, Pneumococcal / diagnosis
  • Pneumonia, Pneumococcal / immunology
  • Streptococcus pneumoniae / immunology


  • Macrophage-Activating Factors