Analysis of the Gene Encoding the Largest Subunit of RNA Polymerase II in Drosophila

Mol Gen Genet. 1989 Jan;215(2):266-75. doi: 10.1007/BF00339727.


We have characterized RpII215, the gene encoding the largest subunit of RNA polymerase II in Drosophila melanogaster. DNA sequencing and nuclease S1 analyses provided the primary structure of this gene, its 7 kb RNA and 215 kDa protein products. The amino-terminal 80% of the subunit harbors regions with strong homology to the beta' subunit of Escherichia coli RNA polymerase and to the largest subunits of other eukaryotic RNA polymerases. The carboxyl-terminal 20% of the subunit is composed of multiple repeats of a seven amino acid consensus sequence, Tyr-Ser-Pro-Thr-Ser-Pro-Ser. The homology domains, as well as the unique carboxyl-terminal structure, are considered in the light of current knowledge of RNA polymerase II and the properties of its largest subunit. Additionally, germline transformation demonstrated that a 9.4 kb genomic DNA segment containing the alpha-amanitin-resistant allele, RpII215C4, includes all sequences required to produce amanitin-resistant transformants.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Drosophila melanogaster / enzymology
  • Drosophila melanogaster / genetics*
  • Genes*
  • Macromolecular Substances
  • Molecular Sequence Data
  • RNA Polymerase II / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid


  • Macromolecular Substances
  • DNA
  • RNA Polymerase II

Associated data

  • GENBANK/M27431