Analysis of the Gene Encoding the Largest Subunit of RNA Polymerase II in Drosophila

Mol Gen Genet. 1989 Jan;215(2):266-75. doi: 10.1007/BF00339727.

Abstract

We have characterized RpII215, the gene encoding the largest subunit of RNA polymerase II in Drosophila melanogaster. DNA sequencing and nuclease S1 analyses provided the primary structure of this gene, its 7 kb RNA and 215 kDa protein products. The amino-terminal 80% of the subunit harbors regions with strong homology to the beta' subunit of Escherichia coli RNA polymerase and to the largest subunits of other eukaryotic RNA polymerases. The carboxyl-terminal 20% of the subunit is composed of multiple repeats of a seven amino acid consensus sequence, Tyr-Ser-Pro-Thr-Ser-Pro-Ser. The homology domains, as well as the unique carboxyl-terminal structure, are considered in the light of current knowledge of RNA polymerase II and the properties of its largest subunit. Additionally, germline transformation demonstrated that a 9.4 kb genomic DNA segment containing the alpha-amanitin-resistant allele, RpII215C4, includes all sequences required to produce amanitin-resistant transformants.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Drosophila melanogaster / enzymology
  • Drosophila melanogaster / genetics*
  • Genes*
  • Macromolecular Substances
  • Molecular Sequence Data
  • RNA Polymerase II / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Macromolecular Substances
  • DNA
  • RNA Polymerase II

Associated data

  • GENBANK/M27431