Fluoride increases superoxide production and impairs the respiratory chain in ROS 17/2.8 osteoblastic cells

PLoS One. 2014 Jun 25;9(6):e100768. doi: 10.1371/journal.pone.0100768. eCollection 2014.


It is known that fluoride produces oxidative stress. Inflammation in bone tissue and an impairment of the respiratory chain of liver have been described in treatments with fluoride. Whether the impairment of the respiratory chain and oxidative stress are related is not known. The aim of this work was to study the effects of fluoride on the production of superoxide radical, the function of the respiratory chain and the increase in oxidative stress in ROS 17/2.8 osteoblastic cells. We measured the effect of fluoride (100 µM) on superoxide production, oxygen consumption, lipid peroxidation and antioxidant enzymes activities of cultured cells following the treatment with fluoride. Fluoride decreased oxygen consumption and increased superoxide production immediately after its addition. Furthermore, chronic treatment with fluoride increased oxidative stress status in osteoblastic cells. These results indicate that fluoride could damage bone tissue by inhibiting the respiratory chain, increasing the production of superoxide radicals and thus of the others reactive oxygen species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Dose-Response Relationship, Drug
  • Electron Transport / drug effects
  • Fluorides / adverse effects*
  • Fluorides / blood
  • Osteoblasts / cytology
  • Osteoblasts / drug effects*
  • Osteoblasts / metabolism*
  • Oxidative Stress / drug effects
  • Oxygen Consumption / drug effects
  • Superoxides / metabolism*
  • Time Factors


  • Superoxides
  • Fluorides

Grant support

This paper was funded by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Grant 112-200801-00462 (http://www.conicet.gov.ar). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.