Hrr25 Kinase Promotes Selective Autophagy by Phosphorylating the Cargo Receptor Atg19

EMBO Rep. 2014 Aug;15(8):862-70. doi: 10.15252/embr.201438932. Epub 2014 Jun 26.

Abstract

Autophagy is the major pathway for the delivery of cytoplasmic material to the vacuole or lysosome. Selective autophagy is mediated by cargo receptors, which link the cargo to the scaffold protein Atg11 and to Atg8 family proteins on the forming autophagosomal membrane. We show that the essential kinase Hrr25 activates the cargo receptor Atg19 by phosphorylation, which is required to link cargo to the Atg11 scaffold, allowing selective autophagy to proceed. We also find that the Atg34 cargo receptor is regulated in a similar manner, suggesting a conserved mechanism.

Keywords: Atg11; Atg19; Atg34; Cvt pathway‐autophagy; Hrr25 kinase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Autophagy
  • Autophagy-Related Proteins
  • Casein Kinase I / physiology*
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • Protein Processing, Post-Translational
  • Protein Transport
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / metabolism*
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Saccharomyces cerevisiae Proteins / physiology*
  • Vesicular Transport Proteins / chemistry
  • Vesicular Transport Proteins / metabolism*

Substances

  • ATG19 protein, S cerevisiae
  • Atg11 protein, S cerevisiae
  • Atg34 protein, S cerevisiae
  • Autophagy-Related Proteins
  • Receptors, Cell Surface
  • Receptors, Cytoplasmic and Nuclear
  • Saccharomyces cerevisiae Proteins
  • Vesicular Transport Proteins
  • Casein Kinase I
  • HRR25 protein, S cerevisiae