Delayed c-fos proto-oncogene expression in the rat hippocampus induced by transient global cerebral ischemia: an in situ hybridization study

Brain Res. 1989 Apr 10;484(1-2):393-8. doi: 10.1016/0006-8993(89)90388-0.


The relative levels of c-fos mRNA in individual neurons of the hippocampal formation of rats is dramatically increased following 20 min of cerebral ischemia induced by 4-vessel occlusion. After 24 h of recirculation, a number of scattered neurons in the dentate hilus became hybridization positive. This effect appeared to peak between 24 and 48 h. A few neurons in the pyramidal cell layer of CA1 expressed c-fos as early as 24 h, but the most intense labeling in this region was seen at 72 h of recirculation. These results correlate well with the known distribution of delayed ischemic necrosis in the brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gene Expression Regulation*
  • Hippocampus / metabolism*
  • Hippocampus / physiopathology
  • Ischemic Attack, Transient / metabolism*
  • Male
  • Nucleic Acid Hybridization
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-fos
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Time Factors


  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-fos
  • RNA, Messenger