Energy-regulated molecules maintain young status in the trophocytes and fat cells of old queen honeybees

Biogerontology. 2014 Aug;15(4):389-400. doi: 10.1007/s10522-014-9509-0. Epub 2014 Jun 28.


Queen honeybees (Apis mellifera) have much longer lifespans than worker bees. Energy-regulated molecules in the trophocytes and fat cells of workers during aging have been determined, but are unknown in queen bees. In the present study, energy-regulated molecules were evaluated in the trophocytes and fat cells of young and old queen bees. Adenosine monophosphate-activated protein kinase α2 (AMPK-α2), phosphorylated AMPK-α2 (pAMPK-α2), and cAMP-specific phosphodiesterases activity increased with aging. The pAMPK-α2/AMPK-α2 ratio and AMPK activity; adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) concentrations; the ADP/ATP ratio and the AMP/ATP ratio; the cyclic adenosine monophosphate concentration; forkhead box protein O expression; Silent information regulator T1 (SirT1) expression and activity; and peroxisome proliferator-activated receptor-α (PPAR-α) expression were not significantly different between young and old queen bees. These results show that energy-regulated molecules maintain a youthful status in the trophocytes and fat cells of queen bees during aging. These cells seem to have longevity-promoting mechanisms and may clarify the secret of longevity in queen bees.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylate Kinase / metabolism
  • Adipose Tissue / metabolism*
  • Animals
  • Bees / metabolism*
  • Cyclic AMP / metabolism
  • Energy Metabolism
  • PPAR alpha / metabolism
  • Phosphoric Diester Hydrolases / metabolism


  • PPAR alpha
  • Cyclic AMP
  • Adenylate Kinase
  • Phosphoric Diester Hydrolases