Concepts in quantitative fluorescence microscopy

Methods Cell Biol. 2014;123:1-18. doi: 10.1016/B978-0-12-420138-5.00001-X.


In recent years, there has been an enormous increase in the publication of spatial and temporal measurements made on fluorescence microscopy digital images. Quantitative fluorescence microscopy is a powerful and important tool in biological research but is also an error-prone technique that requires careful attention to detail. In this chapter, we focus on general concepts that are critical to performing accurate and precise quantitative fluorescence microscopy measurements.

Keywords: Fluorescence; Intensity; Measure; Microscopy; Quantify; Quantitate; Quantitation; Resolution; Sampling.

MeSH terms

  • Algorithms
  • Cells, Cultured
  • Image Processing, Computer-Assisted / methods*
  • Image Processing, Computer-Assisted / standards
  • Microscopy, Fluorescence / methods
  • Microscopy, Fluorescence / standards
  • Signal-To-Noise Ratio
  • Single-Cell Analysis / methods
  • Single-Cell Analysis / standards