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. 2015 Jan;14(1):16-25.
doi: 10.1016/j.jcf.2014.06.001. Epub 2014 Jun 25.

Ibuprofen Rescues Mutant Cystic Fibrosis Transmembrane Conductance Regulator Trafficking

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Ibuprofen Rescues Mutant Cystic Fibrosis Transmembrane Conductance Regulator Trafficking

Graeme W Carlile et al. J Cyst Fibros. .
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Abstract

Background: Small molecules as shown by VX809 can rescue the mislocalization of F508del-CFTR. The aim of this study was to identify correctors with a clinical history and their targets of action.

Methods: CFTR correctors were screened using two F508del-CFTR expressing cell based HTS assays. Electrophysiological studies using CFBE41o(-) and HBE cells and in-vivo mouse assays confirmed CFTR rescue. The target of action was attained using pharmacological inhibitors and siRNA to specific genes.

Results: Ibuprofen was identified as a CFTR corrector. Ibuprofen treatment of polarized CFBE41o(-) monolayers increased the short-circuit current (Isc) response to stimulation. In vivo CF mice treatment with ibuprofen restored the CFTR trafficking. SiRNA knock down of cyclooxygenase expression caused partial F508del-CFTR correction.

Conclusion: These studies show that ibuprofen is a CFTR corrector and that it causes correction by COX-1 inhibition. Hence ibuprofen may be suitable to be part of a future CF combination therapy.

Keywords: Cystic fibrosis; NSAID; Protein folding; Protein trafficking.

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