Spatiotemporal control of fibroblast growth factor receptor signals by blue light

Chem Biol. 2014 Jul 17;21(7):903-12. doi: 10.1016/j.chembiol.2014.05.013. Epub 2014 Jun 26.


Fibroblast growth factor receptors (FGFRs) regulate diverse cellular behaviors that should be exquisitely controlled in space and time. We engineered an optically controlled FGFR (optoFGFR1) by exploiting cryptochrome 2, which homointeracts upon blue light irradiation. OptoFGFR1 can rapidly and reversibly control intracellular FGFR1 signaling within seconds by illumination with blue light. At the subcellular level, localized activation of optoFGFR1 induced cytoskeletal reorganization. Utilizing the high spatiotemporal precision of optoFGFR1, we efficiently controlled cell polarity and induced directed cell migration. OptoFGFR1 provides an effective means to precisely control FGFR signaling and is an important optogenetic tool that can be used to study diverse biological processes both in vitro and in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / metabolism
  • Cell Membrane / radiation effects
  • Cell Movement / radiation effects
  • Cell Polarity / radiation effects
  • Cryptochromes / chemistry
  • HeLa Cells
  • Humans
  • Light*
  • Models, Molecular
  • Optogenetics / methods*
  • Protein Conformation
  • Protein Engineering*
  • Receptors, Fibroblast Growth Factor / chemistry
  • Receptors, Fibroblast Growth Factor / genetics*
  • Receptors, Fibroblast Growth Factor / metabolism*
  • Signal Transduction / genetics*
  • Signal Transduction / radiation effects*
  • Spatio-Temporal Analysis


  • Cryptochromes
  • Receptors, Fibroblast Growth Factor