Oleic Acid, deglycosylated vitamin D-binding protein, nitric oxide: a molecular triad made lethal to cancer

Anticancer Res. 2014 Jul;34(7):3569-78.


Background: Oleic Acid (OA) has been shown to have anticancer properties mediated by interaction with proteins such as α-lactalbumin and lactoferrins. Therefore, we synthesized complexes of OA and Gc protein-derived macrophage activating factor (GcMAF) that inhibits per se cancer cell proliferation and metastatic potential. We hypothesised that OA-GcMAF complexes could exploit the anticancer properties of both OA and GcMAF in a synergistic manner. We postulated that the stimulating effects of GcMAF on macrophages might lead to release of nitric oxide (NO).

Patients and methods: Patients with advanced cancer were treated at the Immuno Biotech Treatment Centre with OA-GcMAF-based integrative immunotherapy in combination with a low-carbohydrate, high-protein diet, fermented milk products containing naturally-produced GcMAF, Vitamin D3, omega-3 fatty acids and low-dose acetylsalicylic acid.

Results: Measuring the tumour by ultrasonographic techniques, we observed a decrease of tumour volume of about 25%.

Conclusion: These observations demonstrate that OA, GcMAF and NO can be properly combined and specifically delivered to advanced cancer patients with significant effects on immune system stimulation and tumour volume reduction avoiding harmful side-effects.

Keywords: Gc protein-derived macrophage activating factor; Oleic acid; human cancer; macrophages; nitric oxide; vitamin D.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspirin / administration & dosage
  • Cholecalciferol / administration & dosage
  • Combined Modality Therapy
  • Dietary Carbohydrates / administration & dosage
  • Dietary Proteins / administration & dosage
  • Drug Synergism
  • Female
  • Humans
  • Immunotherapy
  • Macrophage-Activating Factors / administration & dosage*
  • Macrophage-Activating Factors / chemistry
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Male
  • Middle Aged
  • Neoplasms / diet therapy
  • Neoplasms / drug therapy
  • Neoplasms / metabolism
  • Neoplasms / therapy*
  • Nitric Oxide / metabolism*
  • Oleic Acid / administration & dosage*
  • Oleic Acid / chemistry
  • Vitamin D-Binding Protein / administration & dosage*
  • Vitamin D-Binding Protein / chemistry


  • Dietary Carbohydrates
  • Dietary Proteins
  • Macrophage-Activating Factors
  • Vitamin D-Binding Protein
  • vitamin D-binding protein-macrophage activating factor
  • Cholecalciferol
  • Oleic Acid
  • Nitric Oxide
  • Aspirin