A protein involved in termination of chromosome replication in Bacillus subtilis binds specifically to the terC site

P J Lewis; M T Smith; R G Wake

doi:10.1128/jb.171.6.3564-3567.1989

A protein involved in termination of chromosome replication in Bacillus subtilis binds specifically to the terC site

J Bacteriol. 1989 Jun;171(6):3564-7. doi: 10.1128/jb.171.6.3564-3567.1989.

Authors

P J Lewis¹, M T Smith, R G Wake

Affiliation

¹ Department of Biochemistry, University of Sydney, New South Wales, Australia.

Abstract

The small basic protein encoded by the open reading frame adjacent to the terC site in the Bacillus subtilis chromosome and previously implicated in termination of the replication process was purified. Band retardation assays established that this protein (now called the replication terminator protein, encoded by the rtp gene) binds specifically to a 209-base-pair fragment of DNA within which terC is located.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / genetics*
Bacterial Proteins*
Chromosomes, Bacterial / physiology
Cloning, Molecular
DNA Replication*
DNA, Bacterial / metabolism
DNA-Binding Proteins / physiology*
Genes, Bacterial*
Molecular Weight
Regulatory Sequences, Nucleic Acid*

Substances

Bacterial Proteins
DNA, Bacterial
DNA-Binding Proteins
rtP protein, Bacillus subtilis