Effect of oral consumption of probiotic Lactobacillus planatarum P-8 on fecal microbiota, SIgA, SCFAs, and TBAs of adults of different ages

Nutrition. Jul-Aug 2014;30(7-8):776-83.e1. doi: 10.1016/j.nut.2013.11.018. Epub 2013 Dec 4.

Abstract

Objective: Probiotics may improve intestinal health by modulating intestinal microbiota. However, the age-related variation in response to probiotic consumption is understudied. The aim of this study was to determine the effect of oral consumption of Lactobacillus plantarum P-8 (Lp-8) on human intestinal microflora, secretary immunoglobulin A (SIgA), total bile acids (TBAs), and short-chain fatty acids (SCFAs) of different aged adults.

Methods: Thirty-three recruited individuals, including young (mean age 26 y), middle-aged (mean age 51 y), and elderly (mean age 76 y) volunteers, were given a single daily oral dose of Lp-8 (6 × 10(10) colony forming units) for 4 wk. Fecal samples were collected before starting, during, and after stopping Lp-8 intake. Polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis, quantitative PCR, high-performance liquid chromatography, and enzyme-linked immunosorbent assay were used to detect for fecal microflora, SIgA, TBAs, and SCFAs.

Results: Results showed an increase in Bifidobacterium (P < 0.05) and other beneficial bacteria, whereas Desulfovibrio (P < 0.05) and other opportunistic pathogens decreased after taking Lp-8 for 4 wk. Lp-8 consumption also affected fecal levels of SIgA, TBAs, and SCFAs.

Conclusions: Lp-8 administration could improve human gastrointestinal health. However, some of these effects were transient and gradually disappeared once the intervention was discontinued. Moreover, the extent of these desirable effects was age-related.

Keywords: Fecal microbiota; Gut microbiota; Human; Lactobacillus plantarum; Lp-8; Probiotics.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Bifidobacterium / growth & development
  • Bile Acids and Salts / metabolism*
  • Colon / metabolism
  • Colon / microbiology*
  • Desulfovibrio / growth & development
  • Fatty Acids, Volatile / metabolism*
  • Feces / chemistry
  • Feces / microbiology
  • Humans
  • Immunoglobulin A / metabolism*
  • Lactobacillus*
  • Microbiota*
  • Middle Aged
  • Probiotics*

Substances

  • Bile Acids and Salts
  • Fatty Acids, Volatile
  • Immunoglobulin A