Antioxidative capacity of Lactobacillus fermentum LF31 evaluated in vitro by oxygen radical absorbance capacity assay

Nutrition. Jul-Aug 2014;30(7-8):936-8. doi: 10.1016/j.nut.2013.12.009. Epub 2013 Dec 15.

Abstract

Objective: The aim of this study was to evaluate the performance of the overall antioxidant of Lactobacillus fermentum LF31 bacterium with prebiotic supplement in human colon cultured cells.

Methods: The antioxidant capability of L. fermentum LF31 has been assayed in vitro on human colon adenocarcinoma HT-29 cell line using the oxygen radical absorbance capacity method.

Results: The analysis revealed that the interaction of probiotic strain cells supplemented with a prebiotic exerts a remarkable antioxidant capacity.

Conclusion: The L. fermentum used in the present study exhibited significant in vitro antioxidant capacity, increasing the total antioxidant potential.

Keywords: Antioxidative capacity; Cell culture; ORAC; Probiotic.

MeSH terms

  • Antioxidants / pharmacology*
  • Colon* / metabolism
  • Colon* / microbiology
  • HT29 Cells
  • Humans
  • Lactobacillus fermentum*
  • Prebiotics*
  • Probiotics*
  • Reactive Oxygen Species / metabolism

Substances

  • Antioxidants
  • Prebiotics
  • Reactive Oxygen Species