Artemisia supplementation differentially affects the mucosal and luminal ileal microbiota of diet-induced obese mice

Nutrition. 2014 Jul-Aug;30(7-8 Suppl):S26-30. doi: 10.1016/j.nut.2014.02.007. Epub 2014 Feb 26.


Objective: The gut microbiome has been implicated in obesity and metabolic syndrome; however, most studies have focused on fecal or colonic samples. Several species of Artemisia have been reported to ameliorate insulin signaling both in vitro and in vivo. The aim of this study was to characterize the mucosal and luminal bacterial populations in the terminal ileum with or without supplementation with Artemisia extracts.

Methods: Following 4 wk of supplementation with different Artemisia extracts (PMI 5011, Santa or Scopa), diet-induced obese mice were sacrificed and luminal and mucosal samples of terminal ileum were used to evaluate microbial community composition by pyrosequencing of 16 S rDNA hypervariable regions.

Results: Significant differences in community structure and membership were observed between luminal and mucosal samples, irrespective of diet group. All Artemisia extracts increased the Bacteroidetes to Firmicutes ratio in mucosal samples. This effect was not observed in the luminal compartment. There was high interindividual variability in the phylogenetic assessments of the ileal microbiota, limiting the statistical power of this pilot investigation.

Conclusions: Marked differences in bacterial communities exist depending on the biogeographic compartment in the terminal ileum. Future studies testing the effects of Artemisia or other botanical supplements require larger sample sizes for adequate statistical power.

Keywords: Insulin resistance; Microbiota; Obesity; PMI 5011.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Artemisia*
  • Bacteroidetes
  • Diet
  • Dietary Supplements*
  • Ileum / drug effects*
  • Ileum / microbiology
  • Intestinal Mucosa / drug effects*
  • Intestinal Mucosa / microbiology
  • Mice, Inbred C57BL
  • Mice, Obese
  • Microbiota / drug effects*
  • Obesity / etiology
  • Obesity / microbiology*
  • Phylogeny
  • Plant Extracts / pharmacology*


  • PMI-5011
  • Plant Extracts