3,4-DHPEA-EA from Olea Europaea L. is effective against standard and clinical isolates of Staphylococcus sp

Ann Clin Microbiol Antimicrob. 2014 Jul 1:13:24. doi: 10.1186/1476-0711-13-24.

Abstract

Background: The aim of the present work was to evaluate the antibacterial effect of 3,4-DHPEA-EA (methyl-4-(2-(3,4-dihydroxyphenethoxy)-2-oxoethyl)-3-formyl-2-methyl-3,4-dihydro-2H-pyran-5-carboxylate), a derivate of oleuropein, against a range of Gram-positive bacteria, including ATCC strains, food and clinical isolates.

Methods: The minimum inhibitory concentrations (MICs) of 3,4-DHPEA-EA were determined by the broth microdilution method and the Bioscreen C.

Results: 3,4-DHPEA-EA was effective against ATCC and clinical isolates of Staphylococcus aureus (MIC values between 125 and 250 μg/ml) and ATCC and clinical isolates of Staphylococcus epidermidis (MIC values between 7.81 and 62.5 μg/ml). No significant differences were observed between the two solvents (methanol and DMSO) used to dissolve 3,4-DHPEA-EA.

Conclusions: The results obtained could be used to develop novel therapies for the treatment of skin infections. Further studies need to be performed to elucidate the formation of 3,4-DHPEA-EA by acid hydrolysis of oleuropein in the human stomach.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / pharmacology*
  • Humans
  • Microbial Sensitivity Tests
  • Olea / chemistry*
  • Phenols / isolation & purification
  • Phenols / pharmacology*
  • Phytochemicals / isolation & purification
  • Phytochemicals / pharmacology*
  • Pyrans / isolation & purification
  • Pyrans / pharmacology*
  • Staphylococcus aureus / drug effects*
  • Staphylococcus epidermidis / drug effects*

Substances

  • 3,4-dixydroxyphenylethanol elenolic acid
  • Anti-Bacterial Agents
  • Phenols
  • Phytochemicals
  • Pyrans