Collagen scaffold arrays for combinatorial screening of biophysical and biochemical regulators of cell behavior

Adv Healthc Mater. 2015 Jan 7;4(1):58-64. doi: 10.1002/adhm.201400252. Epub 2014 Jul 2.

Abstract

Arrays of 3D macroporous collagen scaffolds with orthogonal gradations of structural and biomolecular cues are described. Gradient maker technology is applied to create linear biomolecular gradients within microstructurally distinct sections of a single CG scaffold array. The array set up is used to explore cell behaviors including proliferation and regulation of stem cell fate.

Keywords: arrays; collagen; freeze-drying; gradients; mesenchymal stem cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Collagen / chemistry*
  • Fibroblasts / cytology
  • Fibroblasts / metabolism*
  • Humans
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Mice
  • Microfluidic Analytical Techniques* / instrumentation
  • Microfluidic Analytical Techniques* / methods
  • Tissue Array Analysis* / instrumentation
  • Tissue Array Analysis* / methods

Substances

  • Collagen