Validation of video motion-detection scoring of forced swim test in mice

J Neurosci Methods. 2014 Sep 30;235:59-64. doi: 10.1016/j.jneumeth.2014.06.002. Epub 2014 Jul 1.

Abstract

Background: The forced swim test (FST) is used to predict the effectiveness of novel antidepressant treatments. In this test, a mouse or rat is placed in a beaker of water for several minutes, and the amount of time spent passively floating is measured; antidepressants reduce the amount of such immobility. Though the FST is commonly used, manually scoring the test is time-consuming and involves considerable subjectivity.

New method: We developed a simple MATLAB-based motion-detection method to quantify mice's activity in videos of FST. FST trials are video-recorded from a side view. Each pixel of the video is compared between subsequent video frames; if the pixel's color difference surpasses a threshold, a motion count is recorded.

Results: Human-scored immobility time correlates well with total motion detected by the computer (r=-0.80) and immobility time determined by the computer (r=0.83). Our computer method successfully detects group differences in activity between genotypes and different days of testing. Furthermore, we observe heterosis for this behavior, in which (C57BL/6J×A/J) F1 hybrid mice are more active in the FST than the parental strains.

Comparison with existing methods: This computer-scoring method is much faster and more objective than human scoring. Other automatic scoring methods exist, but they require the purchase of expensive hardware and/or software.

Conclusion: This computer-scoring method is an effective, fast, and low-cost method of quantifying the FST. It is validated by replicating statistical differences observed in traditional visual scoring. We also demonstrate a case of heterosis in the FST.

Keywords: Automation; Depression; Forced swim test; Heterosis; Overdominance; Strain differences; Video analysis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Validation Study

MeSH terms

  • Animals
  • Depression / diagnosis*
  • Mice
  • Mice, Inbred C57BL
  • Motion*
  • Motor Activity
  • Neuropsychological Tests*
  • Pattern Recognition, Automated / methods*
  • Software
  • Species Specificity
  • Swimming*
  • Time Factors
  • Video Recording / methods*