Three puncture sites used for in utero electroporation show no significantly different negative impacts during gene transfer into the embryonic mouse brain

Abstract

Although various ways to manipulate genes in vivo exist, in utero electroporation is a widely used technique, especially in the field of neural development due to its many advantages. In this study, we focused on direct comparison between three puncture sites during in utero electroporation on the death rate of embryos, the thickness and the area of cortex, cell differentiation, cell proliferation, cell migration and cell apoptosis. We found no statistical significant differences between the three puncture methods in the death rate of embryos, the thickness and the area of cortex, cell differentiation, cell proliferation, cell migration and cell apoptosis.

Keywords: Differentiation; In utero electroporation; Proliferation; Puncture site; Thickness of cortex.