Polyphenols of selected peach and plum genotypes reduce cell viability and inhibit proliferation of breast cancer cells while not affecting normal cells

Food Chem. 2014 Dec 1:164:363-70. doi: 10.1016/j.foodchem.2014.05.060. Epub 2014 May 20.


Polyphenolic extracts and fractions of selected peach and plum genotypes were evaluated for cell viability and antiproliferation activity in vitro against an estrogen independent MDA-MB-435 and estrogen dependent MCF-7 breast cancer cell lines and one non-cancerous breast line MCF-10A. All extracts showed a phenolic dose-dependent cytotoxic effect against MDA-MB-435, weak activity against MCF-7 and small or no activity against MCF-10A. Genotype phenolic profiles showed varying degrees of polyphenolic mixtures. Fractionation of peach BY00P6653 extracts gave 4 fractions, with fraction F-I (caffeic acid derivatives) showing a strong activity against MDA-MB-435 followed by fraction F-II (anthocyanins). Induced-apoptosis by F-I on MDA-MB-435 was confirmed by Tunnel nuclear staining of cells with apoptotic DNA fragmentation (0-100 μg/mL) with no effects in normal cells (0-200 μg/mL). Selected stone fruit genotypes can be added to the list of fruits with cytotoxic effects against breast cancer cells while not affecting normal cells.

Keywords: Apoptosis; Breast cancer; Polyphenols; Prunus persica; Prunus salicina; Selective cytotoxicity.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Apoptosis / drug effects
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Cell Proliferation / drug effects*
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Estrogens / metabolism
  • Female
  • Fruit / chemistry
  • Fruit / metabolism
  • Genotype
  • Humans
  • In Situ Nick-End Labeling
  • MCF-7 Cells
  • Mammary Glands, Human / drug effects
  • Mammary Glands, Human / metabolism
  • Mammary Glands, Human / pathology
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Polyphenols / isolation & purification
  • Polyphenols / pharmacology*
  • Prunus / chemistry*
  • Prunus / genetics


  • Antineoplastic Agents
  • Antioxidants
  • Estrogens
  • Plant Extracts
  • Polyphenols