Low antibody-dependent cellular cytotoxicity responses in Zambians prior to HIV-1 intrasubtype C superinfection

Debby Basu; Peng Xiao; Zachary Ende; Alfred Bere; William J Britt; Joseph Mulenga; William Kilembe; Susan A Allen; Cynthia A Derdeyn; Eric Hunter

doi:10.1016/j.virol.2014.06.016

Low antibody-dependent cellular cytotoxicity responses in Zambians prior to HIV-1 intrasubtype C superinfection

Virology. 2014 Aug:462-463:295-8. doi: 10.1016/j.virol.2014.06.016. Epub 2014 Jul 5.

Authors

Debby Basu¹, Peng Xiao², Zachary Ende³, Alfred Bere⁴, William J Britt⁵, Joseph Mulenga⁶, William Kilembe⁷, Susan A Allen⁸, Cynthia A Derdeyn⁹, Eric Hunter¹⁰

Affiliations

¹ Emory Vaccine Center at Yerkes National Primate Research Center, 954 Gatewood Road, Atlanta, GA 30329, USA. Electronic address: dbasu@emory.edu.
² Emory Vaccine Center at Yerkes National Primate Research Center, 954 Gatewood Road, Atlanta, GA 30329, USA. Electronic address: peng.xiao@emory.edu.
³ Emory Vaccine Center at Yerkes National Primate Research Center, 954 Gatewood Road, Atlanta, GA 30329, USA. Electronic address: zachary.s.ende@emory.edu.
⁴ Emory Vaccine Center at Yerkes National Primate Research Center, 954 Gatewood Road, Atlanta, GA 30329, USA. Electronic address: alfred.bere@emory.edu.
⁵ University of Alabama - Birmingham, 1600 7th Avenue South, Children׳s Harbor Building 107, Birmingham, AL 35233-1711, USA. Electronic address: WBritt@peds.uab.edu.
⁶ Zambia Emory HIV Research Project (ZEHRP), B22/737 Mwembelelo, Emmasdale, Post Net 412; P/BagE891, Zambia. Electronic address: joseph.mulenga@znbts.gov.zm.
⁷ Zambia Emory HIV Research Project (ZEHRP), B22/737 Mwembelelo, Emmasdale, Post Net 412; P/BagE891, Zambia. Electronic address: wkilembe@rzhrg-mail.org.
⁸ Zambia Emory HIV Research Project (ZEHRP), B22/737 Mwembelelo, Emmasdale, Post Net 412; P/BagE891, Zambia; Department of Global Health, Rollins School of Public Health, Emory University, 1518 Clifton Rd NE, Atlanta, GA 30322, USA; Department of Pathology and Laboratory Medicine, Emory University, 101, Woodruff Circle, Atlanta, GA 30322, USA. Electronic address: SALLEN5@emory.edu.
⁹ Emory Vaccine Center at Yerkes National Primate Research Center, 954 Gatewood Road, Atlanta, GA 30329, USA; Department of Pathology and Laboratory Medicine, Emory University, 101, Woodruff Circle, Atlanta, GA 30322, USA. Electronic address: cderdey@emory.edu.
¹⁰ Emory Vaccine Center at Yerkes National Primate Research Center, 954 Gatewood Road, Atlanta, GA 30329, USA; Department of Pathology and Laboratory Medicine, Emory University, 101, Woodruff Circle, Atlanta, GA 30322, USA. Electronic address: ehunte4@emory.edu.

Abstract

We have previously shown that HIV-1 superinfected Zambian seroconverters mount low binding and neutralizing antibody responses to their primary HIV-1 infecting virus, which could increase susceptibility to re-infection. Here, we investigated if antibody-dependent cellular cytotoxicity (ADCC), a process by which virus-infected cells are killed, was also reduced. Superinfected individuals exhibited low ADCC activity compared to non-superinfected individuals, but similar levels of CMV-reactive binding antibodies, suggesting superinfected individuals are capable of generating and maintaining virus-specific antibodies.

Keywords: ADCC; HIV dual infection; HIV-1 ADCC; HIV-1 superinfection.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

HIV Antibodies / immunology*
HIV Infections / immunology*
HIV Infections / virology*
HIV-1 / immunology*
Humans
Immunity, Cellular*
Superinfection / immunology*

Substances

HIV Antibodies

Abstract

Publication types

MeSH terms

Substances

Grants and funding