Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34

Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):970-5. doi: 10.1107/S2053230X14010449. Epub 2014 Jun 19.


The phage-proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of the 1289 amino-acid gene product 34 (gp34). Different carboxy-terminal parts of gp34 have been produced and crystallized. Crystals of gp34(726-1289) diffracting X-rays to 2.9 Å resolution, crystals of gp34(781-1289) diffracting to 1.9 Å resolution and crystals of gp34(894-1289) diffracting to 3.0 and 2.0 Å resolution and belonging to different crystal forms were obtained. Native data were collected for gp34(726-1289) and gp34(894-1289), while single-wavelength anomalous diffraction data were collected for selenomethionine-containing gp34(781-1289) and gp34(894-1289). For the latter, high-quality anomalous signal was obtained.

Keywords: bacteriophage T4; gene product 34 (gp34).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage T4 / chemistry*
  • Bacteriophage T4 / metabolism
  • Cloning, Molecular
  • Crystallization
  • Crystallography, X-Ray
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression
  • Protein Structure, Tertiary
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Selenomethionine / chemistry*
  • Viral Tail Proteins / chemistry*
  • Viral Tail Proteins / genetics
  • Viral Tail Proteins / metabolism


  • Recombinant Proteins
  • Viral Tail Proteins
  • Selenomethionine