[Use of immunospecific methods for quantitative estimation of microsomal epoxide hydrolase]

Vopr Med Khim. 1989 Jan-Feb;35(1):47-52.
[Article in Russian]

Abstract

Antiserum, specific to rat liver microsomal epoxide hydrolase, was produced. Three methods for quantitative estimation of the epoxide hydrolase, base on the principle of antigen-antiserum immunoprecipitation, were used: electroimmune analysis by Laurell, radial immunodiffusion, enzyme-linked immunoassay. Distinct correlation between specific enzyme activity and its content in microsomal preparation was demonstrated using a simple and stable procedure of radial immunodiffusion. In the enzyme-linked assay conjugate of protein A with alkaline phosphate served as the secondary antibody. Sensitivity of the procedure described was about 1,000-fold higher as compared with radial immunodiffusion method.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Animals
  • Calibration
  • Enzyme-Linked Immunosorbent Assay
  • Epoxide Hydrolases / metabolism*
  • Immunodiffusion
  • Immunoelectrophoresis
  • Microsomes, Liver / enzymology*
  • Rabbits
  • Rats

Substances

  • Epoxide Hydrolases